Removal of estrogenic activity of 4-tert-octylphenol by ligninolytic enzymes from white rot fungi.

نویسندگان

  • Yuuki Tamagawa
  • Hirofumi Hirai
  • Shingo Kawai
  • Tomoaki Nishida
چکیده

4-tert-Octylphenol (4-t-OP) was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. 4-t-OP completely disappeared after 5 days of treatment and the activities of ligninolytic enzymes, laccase and manganese peroxidase (MnP), were detected during this period, thus suggesting that the disappearance of 4-t-OP is related to these extracellular enzymes. Therefore, 4-t-OP was treated with laccase and MnP prepared from white rot fungi cultures. HPLC analysis demonstrated that 4-t-OP completely disappeared in the reaction mixture after 1 h of treatment with either laccase or MnP. Using the yeast two-hybrid assay system, it was also confirmed that laccase and MnP substantially removed the estrogenic activity of 4-t-OP after 1 and 2 h of treatment, respectively. These results strongly demonstrate that ligninolytic enzymes are effective in removing the estrogenic activity of 4-t-OP.

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عنوان ژورنال:
  • Environmental toxicology

دوره 22 3  شماره 

صفحات  -

تاریخ انتشار 2007